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Job Description

We are seeking a highly motivated and detail-oriented Senior Research Scientist with a strong background in cell and molecular biology to join our multidisciplinary team investigating the molecular and cellular mechanisms regulating human megakaryopoiesis and hematopoietic stem cell biology.

The ideal candidate will have extensive hands-on experience with primary ex vivo culture of human hematopoietic stem and progenitor cells, expertise in multicolor flow cytometry Attune NxT, molecular biology and genetic manipulation techniques.

The position offers the opportunity to contribute directly to cutting-edge research aimed at understanding platelet production, stem cell regulation, and translational approaches in hematology.

Please include a cover letter describing relevant experience and research interests with your resume.

Qualifications

+ Ph.D in sciences or related field

+ Minimum five years of experience with scientific investigation.

+ Must have demonstrated outstanding achievement in a field of research

+ Must have contributed to a number of peer-reviewed publications, inventions or the like, at least some of which have had a major impact on advancing the field or discipline.

+ Prior experience successfully securing external grant funding is highly desirable.

+ Preferred:

+ 4–5 years of hands-on laboratory experience in cell biology, preferably with hematopoietic stem cells or primary human cell systems

+ Demonstrated outstanding achievement in a field of research

+ Proven expertise in flow cytometry platforms, including instrument setup, compensation design and analysis (e.g., using FlowJo, FCS Express, or similar software) of complex multicolor panels (≥8 colors).

+ Strong technical skills in protein and nucleic acid analyses (Western blot, qPCR, ELISA, etc.).

+ Demonstrated experience in human hematopoietic stem/progenitor cell isolation, culture, and megakaryocyte-erythroid differentiation including immortalized cell lines.

+ Familiarity with cell signaling pathways, cytokine stimulation assays, and phospho-protein detection.

+ Background in gene silencing (siRNA/shRNA), CRISPR/Cas9 genome editing, lentiviral transduction, and molecular cloning techniques.

+ Excellent analytical, data organization, and troubleshooting skills.

+ Ability to work independently while contributing to a collaborative team environment.

+ Must be able to work as a team member and accept constructive criticism to improve performance.

+ Prior experience studying human megakaryopoiesis, platelet biogenesis, or hematopoietic stem cell signaling.

+ Experience with colony-forming assays (CFU, CFU-Mk) and liquid culture systems for hematopoietic progenitors.

+ Experience with cell signaling pathway analysis (e.g., phospho-flow cytometry, and cytokine-inhibitor based stimulation systems).

+ Experience with gene silencing (siRNA/shRNA) or lentiviral /retroviral transduction, CRISPR/Cas9 editing.

+ Knowledge of immunofluorescence microscopy and image-based analysis for cellular morphology and megakaryocyte maturation assessment.

+ Knowledge of bioinformatics tools for flow cytometry and gene expression analysis (e.g., FlowJo, FCS Express, Prism, etc.).

+ Previous experience in translational hematology or stem cell therapy–related research.

Responsibilities

+ Perform isolation and culture of primary human hematopoietic stem and progenitor cells (CD34⁺) and other defined subsets from mobilized peripheral blood or bone marrow.

+ Execute ex vivo differentiation assays for megakaryocyte and erythroid lineages, including morphological, molecular, and functional characterization.

+ Operate, maintain, and calibrate Attune flow cytometer, including setup-optimize of multicolor panel design for complex immunophenotyping, cell sorting and quantification of hematopoietic subsets including mature megakaryocytes and platelets.

+ Perform methyl cellulose semisolid colony-forming assays (CFU, CFU-Mk) and liquid culture systems for hematopoietic progenitors.

+ Perform protein-based assays, including protein extraction, Western blotting, ELISA, for pathway and signaling studies.

+ Conduct RNA and DNA extractions, RT-qPCR, and basic molecular biology techniques for gene expression and validation studies.

+ Perform siRNA/shRNA, CRISPR/Cas9-mediated gene editing in primary and immortalized cell systems.

+ Maintain accurate and organized experimental records, laboratory notebooks, and sample inventories.

+ Assist in the development and optimization of new protocols for cell signaling, differentiation, and functional assays.

+ Assist with data analysis, figure preparation, and contribute to manuscripts and grant applications.

About Us

Strength through Unity and Inclusion

The Mount Sinai Health System is committed to fostering an environment where everyone can contribute to excellence. We share a common dedication to delivering outstanding patient care. When you join us, you become part of Mount Sinai’s unparalleled legacy of achievement, education, and innovation as we work together to transform healthcare. We encourage all team members to actively participate in creating a culture that ensures fair access to opportunities, promotes inclusive practices, and supports the success of every individual.

At Mount Sinai, our leaders are committed to fostering a workplace where all employees feel valued, respected, and empowered to grow. We strive to create an environment where collaboration, fairness, and continuous learning drive positive change, improving the well-being of our staff, patients, and organization. Our leaders are expected to challenge outdated practices, promote a culture of respect, and work toward meaningful improvements that enhance patient care and workplace experiences. We are dedicated to building a supportive and welcoming environment where everyone has the opportunity to thrive and advance professionally. Explore this opportunity and be part of the next chapter in our history.

About the Mount Sinai Health System:

Mount Sinai Health System is one of the largest academic medical systems in the New York metro area, with more than 48,000 employees working across eight hospitals, more than 400 outpatient practices, more than 300 labs, a school of nursing, and a leading school of medicine and graduate education. Mount Sinai advances health for all people, everywhere, by taking on the most complex health care challenges of our time — discovering and applying new scientific learning and knowledge; developing safer, more effective treatments; educating the next generation of medical leaders and innovators; and supporting local communities by delivering high-quality care to all who need it. Through the integration of its hospitals, labs, and schools, Mount Sinai offers comprehensive health care solutions from birth through geriatrics, leveraging innovative approaches such as artificial intelligence and informatics while keeping patients’ medical and emotional needs at the center of all treatment. The Health System includes more than 9,000 primary and specialty care physicians; 13 joint-venture outpatient surgery centers throughout the five boroughs of New York City, Westchester, Long Island, and Florida; and more than 30 affiliated community health centers. We are consistently ranked by U.S. News & World Report's Best Hospitals, receiving high "Honor Roll" status, and are highly ranked: No. 1 in Geriatrics, top 5 in Cardiology/Heart Surgery, and top 20 in Diabetes/Endocrinology, Gastroenterology/GI Surgery, Neurology/Neurosurgery, Orthopedics, Pulmonology/Lung Surgery, Rehabilitation, and Urology. New York Eye and Ear Infirmary of Mount Sinai is ranked No. 12 in Ophthalmology. U.S. News & World Report’s “Best Children’s Hospitals” ranks Mount Sinai Kravis Children's Hospital among the country’s best in several pediatric specialties. The Icahn School of Medicine at Mount Sinai is ranked No. 11 nationwide in National Institutes of Health funding and in the 99th percentile in research dollars per investigator according to the Association of American Medical Colleges. Newsweek’s “The World’s Best Smart Hospitals” ranks The Mount Sinai Hospital as No. 1 in New York and in the top five globally, and Mount Sinai Morningside in the top 20 globally.

Equal Opportunity Employer

The Mount Sinai Health System is an equal opportunity employer, complying with all applicable federal civil rights laws. We do not discriminate, exclude, or treat individuals differently based on race, color, national origin, age, religion, disability, sex, sexual orientation, gender, veteran status, or any other characteristic protected by law. We are deeply committed to fostering an environment where all faculty, staff, students, trainees, patients, visitors, and the communities we serve feel respected and supported. Our goal is to create a healthcare and learning institution that actively works to remove barriers, address challenges, and promote fairness in all aspects of our organization.

Compensation Statement

Mount Sinai Health System (MSHS) provides a salary range to comply with the New York City Law on Salary Transparency in Job Advertisements. The salary range for the role is $58661 - $150000 Annually. Actual salaries depend on a variety of factors, including experience, education, and hospital need. The salary range or contractual rate listed does not include bonuses/incentive, differential pay or other forms of compensation or benefits.