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Position Objective: Provide services as a Laboratory Technician in support of the overall functions of the TB Imaging Program (TBIP), Division of Intramural Research (DIR) within the National Institute of Allergy and Infectious Diseases (NIAID).

Duties and Responsibilities:

+ Work in a BSL-3 high containment environment.

+ Work in an Animal BSL-3 high containment environment.

+ Assist in conducting experiments to better understand the immune response to Mycobacterium tuberculosis in the macaque animal model.

+ Perform immune cell isolation, cryo-preservation, cryo-recovery and flow cytometric analysis of human blood.

+ Perform immunohistochemical analysis of NHP and human lung tissue specimens, including method development and operating the slide scanner and organizing the resulting image files.

+ Perform a wide variety of procedures and techniques which may include, but are not limited to: cell culture, clinical/diagnostic microbiology, DNA analysis, DNA isolation, DNA purification, DNA sequencing, flow cytometry, gene cloning, manipulation of radioactive isotopes, natural product isolation, organic synthesis, peptide synthesis, protein analysis, RNA isolation, etc. Process primate and murine tissue and sample for downstream applications.

+ Perform flow cytometric cell sorting and/or analysis of research scientist-supplied samples.

+ Maintain detailed records of experiments and their results and prepare the information resulting from these experiments for reports at laboratory-wide, NIH-wide, and for publication.

+ Perform quality control and decontamination of the various laboratory spaces.

+ Generate laboratory records to support laboratory research, and independently performing necessary calculations.

+ Write reports of findings, state methods and procedures, include any modifications employed, specimens and materials involved, and results of experiments.

+ Collect, analyze, and discuss the results of each experiment with the Federal Task Leader (FTL) as part of the planning process for subsequent studies and present these findings in individual weekly meetings with the FTL as well as lab group meetings.

+ Maintain laboratory upkeep and routine maintenance of equipment.

+ Maintain laboratory supplies and equipment including chemicals, biological materials, etc. required to handle biological materials, carcinogens, recombinant protein materials, acids, biological wastes, etc.

Basic Qualifications:

+ BS degree or higher is required.

+ 2 or more years of working experience in a lab, BSL-3 lab and high containment animal facility experience is a plus.

+ Knowledge of operating flow cytometers and step in single cell RNA seq procedures.

+ Good ability to understand and troubleshoot complex equipment.

+ Ability to work independently on computers, software, and with imaging data to organize and create large datasets for analysis and conduct the analysis.

+ Must be able to pass extensive background check and Public Trust 5 clearance.

Minimum Qualifications:

+ Ability to work in a team under supervision in ABSL-3 laboratory environments following SOPs.

+ Ability to communicate effectively, orally and in writing, in a high stress environment with non-technical and technical staff.

+ Detail-oriented and possess strong organizational skills with the ability to prioritize multiple tasks and projects

+ Willing to work flexible hours, rare weekend work possible

*This job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required by this position.

To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

GAP Solutions provides reasonable accommodations to qualified individuals with disabilities. If you need an accommodation to apply for a job, email us at [email protected] . You will need to reference the requisition number of the position in which you are interested. Your message will be routed to the appropriate recruiter who will assist you. Please note, this email address is only to be used for those individuals who need an accommodation to apply for a job. Emails for any other reason or those that do not include a requisition number will not be returned.

Qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, protected veteran status or other characteristics protected by law.

This position is contingent upon contract award.

**Requisition ID:** 7339