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Organization Overview

We study nuclear pore complexes (NPCs), which provide the only known sites for nucleocytoplasmic exchange. Using a variety of techniques and yeast as a model system, we are determining the composition and fine structure of the NPC and relating this to its sites of interactions and reactions with soluble nuclear transport factors. We are also examining associations between NPCs and other components of the nuclear periphery. We hope to gain a better understanding of the part the NPC plays in nuclear regulation and maintenance.

Overview

LINE-1 (L1) retrotransposons are genetic parasites that proliferate in our genomes through a 'copy-and-paste’ mechanism. L1-encoded proteins (ORF1p, ORF2p) preferentially assemble with the L1 RNA that encodes them, producing ribonucleoprotein (RNP) intermediates that are imbued with pathogen associated molecular patterns. These assemblies are poorly understood and heterogeneous in composition, incorporating many other host-derived RNAs and proteins that form abundant cytoplasmic foci. Dysregulated L1 expression is associated with several human diseases - e.g., cancer, autoimmunity, neurodegeneration - and, generally, with aging. L1 therefore represents a promising biomarker opportunity. ORF2p is the ‘business end’ of L1, possessing the requisite endonuclease and reverse transcriptase activities, yet the mechanisms of L1 mobilization remain poorly understood. This is in-part because, even when L1s are naturally expressed, ORF2p is extremely lowly abundant.

The Research Specialist will be responsible for advancing our research team’s L1 analyses – especially around the areas of L1 RNP composition and the _in vitro_ activity of affinity enriched L1 RNPs. This will involve various biochemical and ‘omic techniques. Working knowledge of mass spectrometry and hands-on experience with data collection and bioinformatic analyses is an advantage. The Research Specialist will run some of their own projects semi-independently, in consultation with their direct report and will also assist/support PhD-level researchers in their projects. The Research Specialist may also engage in some team management / administration tasks, but these will be minimal compared to hands-on research.

Responsibilities

Responsibilities include but are not limited to the following:

+ General protein and nucleic acids analyses

+ Human cell culture

+ Mass spectrometry for proteomics

+ L1 project management and some team administration

Qualifications

REQUIRED QUALIFICATIONS:

+ Bachelor's degree required

+ Minimum of 4 years relevant research experience in academic and/or industrial research settings

+ Extensive experience with protein-centric analyses including:

+ Immuno-precipitation

+ SDS-PAGE / general staining

+ Western blotting

+ General protein and nucleic acids biochemistry, theory and techniques

+ Mass spectrometry for proteomics, at least data analysis

+ Nucleic acids sequencing (short and long read), experimental design and data analysis

+ The ability to work (semi)independently to support PhD-level scientists

PREFERRED QUALIFICATIONS:

+ Knowledge and experience working with LINE-1 retrotransposons

+ Prior industry experience

+ Prior expreience with advanced protein research, including mass spectrometry, will be prioritized over sequencing

The Rockefeller University is an equal opportunity employer – veterans/individuals with disabilities. Qualified applicants will receive consideration for employment without regard to characteristics protected by applicable local, state or federal law, including but not limited to disability and protected veteran status.

The salary of the finalist selected for this role will be set based on various factors, including but not limited to organizational budgets, qualifications, experience, education, licenses, specialty, and training. The hiring range provided represents The Rockefeller University's good faith and reasonable estimate of the range of possible compensation at the time of posting.

Compensation Range: Min

USD $70,000.00/Yr.

Compensation Range: Max

USD $80,000.00/Yr.