• Research Technologist

    Johns Hopkins University (Baltimore, MD)


    Welcome to the Watanabe Lab at Johns Hopkins University – A vibrant and pioneering hub of neurobiology research, where we delve into the intricate world of synaptic transmission. As you consider the possibility of joining our team as a **_Research Technologist_** , allow us to paint a vivid picture of who we are:

     

    + Our central focus is synaptic transmission, unraveling the mechanisms that underlie communication between neurons.

    + We explore the pathways that sustain neurotransmission and how other cell types and states influence this.

    + We are a growing and evolving lab with members ranging from undergraduates, research technologists, graduate students, post-docs, and research associates.

    + We employ a variety of techniques from electron microscopy, super resolution and live imaging, and electrophysiology.

    Our Lab Culture

    + Beyond traditional academic hierarchies, we cultivate an inclusive and collaborative culture that celebrates diversity.

    + We believe in fostering an environment where every voice is heard, regardless of background or experience.

    + We embrace a flat organizational structure, where ideas are valued based on their merit, and collaborative efforts drive us forward.

    + Your unique perspective will enrich our collective understanding, and your contributions will be celebrated as essential pieces of our research puzzle.

    + We value innovation and adaptability in our research methods, ideas, and interactions.

    Your Role as a Research Technologist in our Lab

    + Our research extends into the dynamic role of microglia, the brain's immune cells, in shaping and modulating synaptic connections.

    + As a Research Technologist on this project, you will be at the heart of discovery, contributing to the understanding of the fundamental functions of microglia at the synapse.

    + This role serves as a bridge between the minor and significant purposes of our lab, as you'll contribute both to the day-to-day operations and the grand narrative of our science.

    + You will have the opportunity to be an active participant in our lab, conducting experiments, collecting and analyzing data, and translating observations into meaningful insights.

    + You will collaborate closely with a diverse team of scientists, from enthusiastic students to established post-docs and research associates.

    + Your skill set will be honed, providing you with opportunities to engage in discussions and foster your professional development.

    Specific Duties & Responsibilities

    + Collaborate with senior researchers to design and plan experiments.

    + Independently conduct experiments while adhering to established protocols and safety guidelines.

    + Producing lentiviruses for gene manipulation and DNA cloning for vector construction.

    + Isolate and culture microglia from brain tissue.

    + Employ imaging techniques (immunohistochemistry, confocal, and electron microscopy) to visualize neural structures and microglia in both cultured cells and tissue.

    + Record and analyze data, utilizing software tools for statistical analysis and visualization.

    + Organize, prepare, and manage laboratory reagents, ensuring equipment and materials are ready for experiments.

    + Contribute to maintaining a clean and safe working environment, following proper waste disposal procedures.

    + Keep detailed and accurate records of experimental procedures, observations, and outcomes.

    + Assist in preparing research findings for presentations at lab meetings, conferences, and publications.

    + Collaborate effectively with fellow researchers, sharing insights, troubleshooting, and contributing to a dynamic scientific dialogue.

    + Communicate experimental results clearly in discussions, presentations, and written reports.

    + Embrace the lab's inclusive culture by fostering a collaborative and respectful environment.

    Knowledge, Skills, and Abilities

    + Experience in cell culture methods, including primary cultures.

    + Knowledge of immunostaining and confocal microscopy.

    + Familiarity with molecular biology techniques such as PCR, cloning, and DNA Maxi-preps.

    + Ability to troubleshoot experimental protocols and identify potential technical issues.

    + Strong analytical skills to interpret complex data and contribute to scientific discussions.

    + Detail-oriented approach for precise documentation of experiments, protocols, and results.

    + Excellent time management skills to handle multiple tasks, prioritize, and meet project deadlines.

    + Effective interpersonal skills to collaborate with a diverse team and contribute to a harmonious work environment.

    + Adaptable mindset to embrace new challenges and pivot research directions as needed.

    Minimum Qualifications

    + Bachelor's Degree in cell biology, molecular biology, chemistry, or related field.

    + Some related post-undergraduate work experience preferred.

    + Some related laboratory work experience preferred.

    + Additional education may substitute for required experience, and additional related experience may substitute for required education beyond a High School Diploma/Graduation Equivalent, to the extent permitted by the JHU Equivalency formula.

    Preferred Qualifications

    + Training in biosafety protocols and adeptness in handling viral vectors within laboratory environments.

    + Experience in animal handling and proper care of research animals.

    + Research background in the realm of neurobiology.

    + Experience in electron microscopy techniques.

     

    Classified Title: Research Technologist

    Role/Level/Range: ACRO40/E/02/CC

    Starting Salary Range: $15.70 - $26.25/HRLY ($42,000.00 Annually/Targeted; Commensurate with experience)

     

    Employee group: Full Time

     

    Schedule: Monday-Friday, 8:30 AM - 5:00 PM

     

    FLSA Status: Non-Exempt

     

    Department name: SOM, Cell Biology

    Personnel area: School of Medicine

    The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range.

     

    Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/.

     

    Equal Opportunity Employer

     

    All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

    EEO is the Law

    https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf