• Research Technologist - #Staff

    Johns Hopkins University (Baltimore, MD)


    We are seeking a **_Research Technologist_** to work in the Coyne Lab in the School of Medicine. The Coyne Lab uses induced pluripotent stem cell (iPSC) derived neuron (iPSN) and postmortem human tissue models to understand the mechanistic basis and pathophysiologic consequences of impaired nuclear pore complex (NPC) and nuclear envelope surveillance and homeostasis in neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD).

     

    The Research Technologist will assist with various projects in the lab and primarily be responsible for the maintenance and differentiation of iPSNs, processing of samples for western blot and qRT-PCR, and carrying out western blots and qRT-PCR experiments. The technology may also assist with immunostaining and confocal imaging as required. We are looking for a candidate with a basic understanding of techniques and a foundation in the underlying science. The individual will work independently to carry out daily tasks with oversight from the PI.

     

    The Research Technologist will participate in planning experiments and evaluating/interpreting test results utilizing knowledge of research laboratory procedures and practices. The individual will use independent judgment in the selection, development, performance and evaluation of research methods and operation of appropriate equipment.

    Specific Duties and Responsibilities

    + Follow established procedures or protocols and utilize aseptic technique to perform laboratory experiments which include: iPSN culture (passaging and maintenance), qRT-PCR, western blotting, cloning and/or subcloning, immunostaining, and other basic cell and molecular biology techniques.

    + Use various software applications, such as Excel spreadsheets, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble and/or format data.

    + Participate in the organization and operation of the laboratory.

    + Maintain written records and electronic databases.

    + Prepare solutions and reagents following standard laboratory formulas and procedures.

    + Plan and execute a defined set of experiments following discussion with the principal investigator.

    + Use universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.

    + Complete annual university biohazard/universal precaution safety training, as appropriate.

    + Monitor biohazard safety standards and proper handling of potentially hazardous chemical and biological agents in the workplace.

    Minimum Qualifications

    + Bachelor's Degree in Biology, Chemistry or related field.

    + Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.

    Preferred Qualifications

    + Some knowledge of, or experience with, aseptic mammalian cell culturing.

    + Basic understanding of, or experience with, western blotting, qRT-PCR, or immunostaining.

     

    Classified Title: Research Technologist

    Role/Level/Range: ACRO40/E/02/CC

    Starting Salary Range: $15.70 - $26.25 HRLY ($36,000 targeted; Commensurate w/exp.)

     

    Employee group: Full Time

     

    Schedule: M-F/40

     

    FLSA Status: Non-Exempt

     

    Department name: Neurodegenerative and Injury Program

    Personnel area: School of Medicine

    The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range.

     

    Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/.

     

    Equal Opportunity Employer

     

    All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

    EEO is the Law

    https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf