• Scientist

    Insight Global (Cambridge, MA)


    Job Description

    We are seeking a scientist to join our Systems Immunology Lab within Inflammation & Immunology Research Unit for a 6 month contract, with likely extensions. The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team.

     

    You will generate custom lentiviral libraries and maintain extensive cell cultures. Generate edited pools of cells via lentiviral transduction and arrayed electroporation. Generate single-cell data via several commercial workflows, including from 10x Genomics. Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale.

     

    We are a company committed to creating diverse and inclusive environments where people can bring their full, authentic selves to work every day. We are an equal opportunity/affirmative action employer that believes everyone matters. Qualified candidates will receive consideration for employment regardless of their race, color, ethnicity, religion, sex (including pregnancy), sexual orientation, gender identity and expression, marital status, national origin, ancestry, genetic factors, age, disability, protected veteran status, military or uniformed service member status, or any other status or characteristic protected by applicable laws, regulations, and ordinances. If you need assistance and/or a reasonable accommodation due to a disability during the application or recruiting process, please send a request to [email protected] learn more about how we collect, keep, and process your private information, please review Insight Global's Workforce Privacy Policy: https://insightglobal.com/workforce-privacy-policy/.

     

    Skills and Requirements

     

    - Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field

     

    -3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry.

     

    - Previous experience working with Diverse Cell Types

     

    - Experience with Molecular biology techniques *qPCR

     

    - Experience in Gene Editing - direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.