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Job Description

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty.

The Division of Medical Genetics has an outstanding opportunity for a highly skilled Research Scientist/Engineer 3** **(FULL-TIME) in the laboratory of Mary-Claire King PhD.

Field of Research:

Human genetics, long-read RNA sequencing, stem cell differentiation, protein chemistry.

Purpose of the research project(s):

This individual will work in a team effort as part of the King Laboratory to use RNA sequencing and protein profiling to study inherited cancers and neuropsychiatric disorders. This position requires a strong background in long read RNA sequencing, stem cell growth, and differentiation protocols, protein production and purification, and devising and testing novel approaches for applying these approaches.

This position is experimental biology and requires full-time in-person work at the Health Sciences Building, K wing. Remote work is not possible for this position.

**Research Sponsor/Stakeholders:** NIH

DUTIES AND RESPONSIBILITIES

_Perform direct RNA sequencing in patient derived neuronal cells (45%)_

• Stem cell tissue culture growth and differentiation protocols, cell transduction and transfection.

• Preparation and quality control of DNA and RNA samples.

• Construction of Direct RNA sequencing libraries and Nanopore sequencing.

• Perform routine analysis of data and assist with troubleshooting and data quality control

• Perform computational analysis of RNA reads.

_Developing tools to enable Nanopore profiling of full-length proteins (45%)_

• Molecular biology techniques such as cloning, PCR, and sequencing (Sanger, short and long read)

• Cloning vectors for protein expression

• Protein purification using bacterial overexpression systems.

• Prepare samples for small molecule mass spectrometry and nanopore profiling.

• Perform routine analysis of data and assist with troubleshooting and data quality control

• Assist with computational analysis of small molecule mass spectrometry and nanopore profiling data

_Additional duties (10%)_

• Present analysis findings at group meetings and national conferences, as appropriate.

• Provide status reports to the Principal Investigator on status of projects.

• Perform related duties as required.

MINIMUM REQUIREMENTS

• Bachelor’s degree in Biology, Biochemistry, Microbiology or related Life Sciences field and four years of relevant experience.

_Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration._

A** **D** **DITIONAL REQUIREMENTS

• Four or more years of experience in stem cell tissue culture, RNA sequencing, expressing and purifying proteins

• Proficient in Nanopore long read DNA and RNA alignment and variant calling tools.

DESIRED QUALIFICATIONS

• PhD in Stem Cell Sciences, Genetics/Genomics, Biology, Biochemistry or equivalent.

• Must be self-motivated and able to take initiative, work independently, and learn new techniques.

• Ability to work, collaborate and communicate well within a multidisciplinary team.

• Strong organizational abilities and laboratory skills, including the ability to keep detailed records of procedures performed and sample tracking.

• Strong written and oral communication skills.

\#UWDeptMedicineJobs

Compensation, Benefits and Position Details

Pay Range Minimum:

$72,288.00 annual

Pay Range Maximum:

$132,612.00 annual

Other Compensation:

-

Benefits:

For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-uw-staff/

Shift:

First Shift (United States of America)

Temporary or Regular?

This is a regular position

FTE (Full-Time Equivalent):

100.00%

Union/Bargaining Unit:

UAW Research

About the UW

Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world.

UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty.

Our Commitment

The University of Washington is proud to be an affirmative action and equal opportunity employer (https://hr.uw.edu/eoaa/) . All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, national origin, age, protected veteran or disabled status, or genetic information.

To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [email protected] .

Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law (https://app.leg.wa.gov/RCW/default.aspx?cite=28B.112.080) .

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.