- Mount Sinai Health System (New York, NY)
- …also include deep biochemical investigation involving mass spectrometry as well as protein purification and assessment of phosphorylation status using western ... blot. Finally using single cell RNA sequencing and using CYTOF technology you will perform in depth immunological characterization of IFN-I responsiveness and other inflammatory markers in these disease settings. **_Requirements:_** Ph.D. degree in related… more
- University of Rochester (Rochester, NY)
- …the supply sterilized and the cell lines properly stored - Performing the protein expression and purification - Train graduate and undergraduate students in ... are currently seeking a skilled Lab Technician specializing in Protein Expression, Cell, and Tissue Culture to join our...team. This role involves the maintenance and execution of protein expression, cell, and tissue culture experiments. The ideal… more
- Mount Sinai Health System (New York, NY)
- …structure research, completed within the last three years. + Proficiency in protein expression and purification in eukaryotic and prokaryotic systems. + ... Comprehensive training in structural biology techniques such as cryo-EM and protein crystallization. + An opportunity to contribute to high-impact research projects… more
- New York University (New York, NY)
- …publications, and background knowledge in: i) Experiences in construct design, cloning, protein expression and purification ; in vitro RNA transcription and ... post-doctoral associate to perform interdisciplinary work on studying RNA structure, RNA- protein interactions, and dynamics, with a molecular glue focus. Successful… more
- University of Rochester (Rochester, NY)
- …fluorescent imaging of cargoes in neuronal processes. DNA isolation and purification , lentivirus purification , protein isolation, gel electrophoresis, ... immunofluorescent analyses and animal husbandry. Opportunities to learn whole cell and optical electrophysiology to quantify synaptic transmission. **ESSENTIAL FUNCTIONS** + Perform isolation and in-vitro culture of hippocampal and cortical neurons from mouse… more
- Regeneron Pharmaceuticals (Tarrytown, NY)
- …designs and screening protocols. + Performing basic molecular biology and protein analysis techniques (Cloning, SDS PAGE analysis, Western blotting, ELISA, DNA ... extractions, PCR, quantitative PCR, protein chromatography, etc.) + Working closely with bioinformatics team...is required. Experience with mammalian cell culture, manipulation and purification of viruses or proteins, DNA isolation from tissue… more
- University of Rochester (Rochester, NY)
- …mouse colony maintenance, genotyping, histology. Tissue dissection, cell isolation and purification , cell culture procedures and genetic manipulation of cells. RNA ... isolation and RT-QPCR, protein isolation, gel electrophoresis, image capture and analysis (CX5),...analysis of cell division and survival, gene expression analysis, protein expression analysis, treatment of mice and analysis of… more
- Mount Sinai Health System (New York, NY)
- …culture, transfection, AAV transduction, miniprep and maxiprep, AAV isolation and purification , IVIS imaging, proficiency in q-PCR, RT-PCR, RNA extraction, cDNA ... synthesis, DNA/RNA gels, quantification, western blot, immunocytochemistry, fluorescence microscope, agarose gel electrophoresis, spectrophotometer, Animal Handling: Mice handling, intraperitonial injection, Tissue processing, bone marrow extraction to derive… more