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The Department of Neurology is seeking a **_Research Technologist_** to work in a molecular virology lab. In this molecular virology (HIV-1, biosafety level 2) laboratory the candidate will utilize their solid foundation in basic laboratory techniques and experience with molecular biology and primary cell culture which requires a high level of rigor and reproducibility. Capable of exercising independent judgment while participating in the planning of experiments and interpreting results. The projects require care in data collection, preparation of reports and interfacing with sponsors. Works effectively as a team-member and exhibits excellent interpersonal, organizational, and self-management skills. Will provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.

Specific Duties & Responsibilities

+ Mouse breeding and husbandry.

+ Handling and performing blood draw and injections on mice infected with viral pathogens.

+ Isolation of peripheral blood lymphocytes and monocytes from human blood.

+ Perform in vitro infection studies with HIV-1.

+ Performs general molecular biology techniques such as cloning, qPCR, and Western blotting.

+ Performs specialized techniques including multicomponent single-cell labeling, confocal and high-content microscopy, and multi-color flow cytometry.

+ Experience and or interest in conducting single-cell bioinformatics is desirable.

Additional Knowledge, Skills and Abilities

+ Familiarity with isolation of PBMCs and culture of primary human cells; sterile technique.

+ Must be willing to work under Biohazard Level 2 Safety conditions with HIV-1.

+ Must display willingness to learn and implement new technologies.

+ Ability for self-management, integrity and professionalism.

Minimum Qualifications

+ Bachelor's Degree in Biology, Chemistry or related field.

+ Occasional irregular work hours consistent with research needs related to the in vivo studies with mice is required.

+ Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.

Preferred Qualifications

+ Some related post-undergraduate work experience preferred

Classified Title: Research Technologist

Role/Level/Range: ACRO40/E/02/CC

Starting Salary Range: $15.70 - $26.25 HRLY (Commensurate w/exp.)

Employee group: Full Time

Schedule: M-F 8:30 am - 5:00 pm

FLSA Status: Non-Exempt

Department name: SOM Neuro Neuroimmunology

Personnel area: School of Medicine

The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range.

Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/.

Equal Opportunity Employer

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

EEO is the Law

https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf