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The Department of Medicine, Division of Gastroenterology is seeking a casual **_Research Specialist_** to work in a laboratory focusing on the biology of vagal sensory neurons in health and disease. The Research Specialist will utilize a solid foundation in basic laboratory techniques to maintain all animal protocols, mouse colonies, laboratory equipment, reagent inventories, and assist in routine experimental work like DNA isolation, PCR, cell culture, live animal surgeries, and animal management. The Research Specialist should have experience with animal handling and should be capable of following protocols to carry out basic animal surgeries as well as cell culture experiments.

Specific Duties and Responsibilities

+ Handling mice and performing injections.

+ Organizing and executing studies of mouse behavior.

+ Surgical dissection of mouse tissues and performing frozen tissue sectioning.

+ Immunostaining.

+ Fluorescent in-situ RNA hybridization using standard kits.

+ Confocal and non-confocal fluorescence microscopy.

+ Discussing experimental protocols and data with the faculty investigator.

+ Genotyping of various transgenic animals.

+ Colony management and timed matings of defined transgenic animal strains.

+ Routine animal survival surgeries for injecting drugs/viruses/dyes into the vagus nerve and gastrointestinal tract of animals.

+ Applying previous knowledge to troubleshoot experiments.

+ Keeping a thorough and organized laboratory notebook to document experiments.

+ Maintaining an orderly laboratory workspace.

+ Ensuring laboratory efficiency by following set protocols for lab cleanliness, organization, and safety regulations.

+ Monitoring inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.

+ Exercises professional and scientific judgment and assumes responsibility for decisions, consequences, and results. Communicates with lab members to understand the biology of sensation of the GI tract by the vagus nerve.

+ Exchanges routine information in an appropriate manner requiring good oral and written communication skills. Has operating knowledge of the personal computer to help maintain the mouse colony database and to communicate with senior lab personnel on planning various experiments.

+ Other duties as assigned.

Minimum Qualifications

+ Bachelor's Degree in Biology, Chemistry or related field.

+ One year of laboratory experience performing relevant techniques.

+ Additional education may substitute for required experience and additional related experience may substitute for required education beyond high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.

Classified Title: Research Specialist

Role/Level/Range: ACRP/03/MA

Starting Salary Range: $17.11 - $29.90 HRLY ($20,000 targeted; Commensurate w/exp.)

Employee group: Casual / On Call

Schedule: M-F Varies

FLSA Status: Non-Exempt

Department name: SOM DOM Gastroenterology

Personnel area: School of Medicine

The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range.

Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/.

Equal Opportunity Employer

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

EEO is the Law

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