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Job Description

We are seeking a highly motivated, detail-oriented Senior Research Scientist with a strong background in cell and molecular biology to join our multidisciplinary research team. Our group investigates the molecular and cellular mechanisms regulating human megakaryopoiesis, hematopoietic stem cell biology, and microenvironmental interactions.

The ideal candidate will have extensive hands-on experience with ex vivo culture of primary human hematopoietic stem and progenitor cells, strong technical expertise in multicolor flow cytometry using the Attune NxT platform, and proficiency in molecular biology and genetic manipulation techniques. This position provides an opportunity to contribute directly to cutting-edge research focused on platelet production, stem cell regulation, cell–cell interactions, and translational studies in hematology.

Please include a cover letter describing relevant experience and research interests with your resume.

Qualifications

+ Ph.D in sciences or related field

+ Minimum five years of experience with scientific investigation.

+ Must have demonstrated outstanding achievement in a field of research

+ Must have contributed to a number of peer-reviewed publications, inventions or the like, at least some of which have had a major impact on advancing the field or discipline.

+ Prior experience successfully securing external grant funding is highly desirable.

+ Preferred:

+ 4–5 years of hands-on laboratory experience in cell biology, ideally involving hematopoietic stem cells or primary human cell systems.

+ Basic experience with handling laboratory mice, including injections, euthanasia, and tissue or bone marrow collection.

+ Proven expertise in flow cytometry, including instrument setup, multicolor compensation, and data analysis (e.g., FlowJo, FCS Express) for panels ≥8 colors.

+ Strong technical skills in protein and nucleic acid analysis (Western blotting, qPCR, ELISA, cytokine stimulation assays, etc.).

+ Demonstrated experience with human hematopoietic stem/progenitor cell isolation, culture, and megakaryocyte/erythroid differentiation, including work with immortalized cell lines.

+ Experience with siRNA/shRNA, CRISPR/Cas9 gene editing, lentiviral transduction, and basic molecular cloning.

+ Excellent analytical abilities, organizational skills, and troubleshooting capabilities.

+ Ability to work both independently and collaboratively within a diverse research team.

+ Receptiveness to feedback and commitment to continuous technical and scientific improvement.

+ Prior experience studying human megakaryopoiesis, platelet biogenesis, or hematopoietic stem cell signaling.

+ Experience performing colony-forming assays (CFU, CFU-Mk) and liquid hematopoietic culture systems.

+ Experience with signaling pathway analyses (e.g., intracellular-flow cytometry, cytokine or inhibitor-based stimulation).

+ Experience with gene silencing approaches or viral transduction systems (lentiviral/retroviral) and CRISPR/Cas9 editing.

+ Knowledge of immunofluorescence microscopy and image-based analysis for assessing cellular morphology and megakaryocyte maturation.

+ Familiarity with bioinformatics tools for cytometry and gene-expression analysis (FlowJo, FCS Express, Prism, etc.).

+ Previous experience in translational hematology or stem cell therapy–related research.

+ Experience performing mouse models of hematologic disease, including dosing, monitoring, and ex vivo analysis of harvested tissues.

Responsibilities

+ Isolate and culture primary human hematopoietic stem and progenitor cells (CD34⁺) and other subsets from mobilized peripheral blood or bone marrow.

+ Perform ex vivo differentiation assays for megakaryocyte and erythroid lineages, including morphological, molecular, and functional analyses.

+ Perform basic mouse handling for hematology-related experiments, including injections, treatments, tissue/bone marrow harvesting, and sample processing, in compliance with institutional animal care and use guidelines.

+ Operate, maintain, and calibrate the Attune NxT flow cytometer; design, optimize, and troubleshoot multicolor panels for complex immunophenotyping, cell sorting, and quantitative analysis of hematopoietic subsets including mature megakaryocytes and platelets.

+ Conduct methylcellulose-based semisolid colony-forming assays (CFU, CFU-Mk) and liquid culture systems for hematopoietic progenitors.

+ Perform protein-based assays (protein extraction, Western blotting, ELISA) for signaling and pathway analyses.

+ Conduct RNA and DNA extractions, RT-qPCR, and standard molecular biology procedures for gene expression and validation studies.

+ Utilize gene-silencing and genome-editing approaches, including siRNA/shRNA, CRISPR/Cas9, and lentiviral transduction in primary and immortalized cells.

+ Maintain accurate, well-organized laboratory notebooks, records, and sample inventories.

+ Assist in developing, optimizing, and standardizing protocols for cell signaling, differentiation, and functional assays.

+ Support data analysis, figure preparation, and contribute to scientific manuscripts and grant applications.

About Us

Strength through Unity and Inclusion

The Mount Sinai Health System is committed to fostering an environment where everyone can contribute to excellence. We share a common dedication to delivering outstanding patient care. When you join us, you become part of Mount Sinai’s unparalleled legacy of achievement, education, and innovation as we work together to transform healthcare. We encourage all team members to actively participate in creating a culture that ensures fair access to opportunities, promotes inclusive practices, and supports the success of every individual.

At Mount Sinai, our leaders are committed to fostering a workplace where all employees feel valued, respected, and empowered to grow. We strive to create an environment where collaboration, fairness, and continuous learning drive positive change, improving the well-being of our staff, patients, and organization. Our leaders are expected to challenge outdated practices, promote a culture of respect, and work toward meaningful improvements that enhance patient care and workplace experiences. We are dedicated to building a supportive and welcoming environment where everyone has the opportunity to thrive and advance professionally. Explore this opportunity and be part of the next chapter in our history.

About the Mount Sinai Health System:

Mount Sinai Health System is one of the largest academic medical systems in the New York metro area, with more than 48,000 employees working across eight hospitals, more than 400 outpatient practices, more than 300 labs, a school of nursing, and a leading school of medicine and graduate education. Mount Sinai advances health for all people, everywhere, by taking on the most complex health care challenges of our time — discovering and applying new scientific learning and knowledge; developing safer, more effective treatments; educating the next generation of medical leaders and innovators; and supporting local communities by delivering high-quality care to all who need it. Through the integration of its hospitals, labs, and schools, Mount Sinai offers comprehensive health care solutions from birth through geriatrics, leveraging innovative approaches such as artificial intelligence and informatics while keeping patients’ medical and emotional needs at the center of all treatment. The Health System includes more than 9,000 primary and specialty care physicians; 13 joint-venture outpatient surgery centers throughout the five boroughs of New York City, Westchester, Long Island, and Florida; and more than 30 affiliated community health centers. We are consistently ranked by U.S. News & World Report's Best Hospitals, receiving high "Honor Roll" status, and are highly ranked: No. 1 in Geriatrics, top 5 in Cardiology/Heart Surgery, and top 20 in Diabetes/Endocrinology, Gastroenterology/GI Surgery, Neurology/Neurosurgery, Orthopedics, Pulmonology/Lung Surgery, Rehabilitation, and Urology. New York Eye and Ear Infirmary of Mount Sinai is ranked No. 12 in Ophthalmology. U.S. News & World Report’s “Best Children’s Hospitals” ranks Mount Sinai Kravis Children's Hospital among the country’s best in several pediatric specialties. The Icahn School of Medicine at Mount Sinai is ranked No. 11 nationwide in National Institutes of Health funding and in the 99th percentile in research dollars per investigator according to the Association of American Medical Colleges. Newsweek’s “The World’s Best Smart Hospitals” ranks The Mount Sinai Hospital as No. 1 in New York and in the top five globally, and Mount Sinai Morningside in the top 20 globally.

Equal Opportunity Employer

The Mount Sinai Health System is an equal opportunity employer, complying with all applicable federal civil rights laws. We do not discriminate, exclude, or treat individuals differently based on race, color, national origin, age, religion, disability, sex, sexual orientation, gender, veteran status, or any other characteristic protected by law. We are deeply committed to fostering an environment where all faculty, staff, students, trainees, patients, visitors, and the communities we serve feel respected and supported. Our goal is to create a healthcare and learning institution that actively works to remove barriers, address challenges, and promote fairness in all aspects of our organization.

Compensation Statement

Mount Sinai Health System (MSHS) provides a salary range to comply with the New York City Law on Salary Transparency in Job Advertisements. The salary range for the role is $58661 - $150000 Annually. Actual salaries depend on a variety of factors, including experience, education, and hospital need. The salary range or contractual rate listed does not include bonuses/incentive, differential pay or other forms of compensation or benefits.